纳滤选择性分离丁二酸模拟发酵液
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摘要
生物法制备的丁二酸发酵液中含有残留的底物(葡萄糖)、甲酸、乙酸及二价无机离子(Mg2+、SO42-)等杂质,不利于分离纯化.采用截留相对分子质量为160的纳滤膜LNG-NF-016对丁二酸模拟发酵液进行分离,考察了pH、操作压力、MgSO4浓度、丁二酸浓度、葡萄糖浓度、料液体积流量、操作温度等因素对纳滤分离性能的影响.纳滤分离的选择性由丁二酸的解离状态决定:模拟发酵液调节至pH 3.0,丁二酸未解离,可将其与葡萄糖、MgSO4分离,丁二酸的透过率达98%,葡萄糖、MgSO4的截留率均>85%.随后将透过液调节至pH 6.0,丁二酸形成二价阴离子,可将其与单价的甲酸盐、乙酸盐分离,丁二酸截留率达90%,丁二酸被浓缩至121 g/L,而膜截留液中的甲酸、乙酸浓度均<5 g/L.同一型号纳滤膜可实现模拟发酵液中丁二酸与副产一元有机酸、底物及多价无机离子的选择性分离.
Many impurities existed in succinic acid fermentation broth,such as by-product organic acids(formic acid,acetic acid),residual substrate(glucose) and divalent ion(Mg2+,SO42-),which was unfavorable to purification.Separation of succinic acid from simulated fermentation broth by nanofiltration membrane LNG-NF-016(MWCO 160) was studied,For this purpose,the effect of operating parameters such as pH in the feed solution,operation pressure,MgSO4 concentration,succinic acid concentration,glucose concentration,cross-flow rate and operation temperature on nanofiltration was investigated using an experimental design technique.The selectivity of nanofiltration depended on the degree of dissociation of succinic acid.At pH about 3.0,undissociated succinic acid could be separated from glucose,and MgSO4with succinic acid permeation of 98% and glucose and MgSO4 with rejection more than 85%.The divalent succinate could be separated from acetate and formate at pH about 6.0,succinic acid rejection of 90%,succinic acid concentration about 121 g/L in retentate,formic acid and acetic acid concentrations below 5 g/L.Succinic acid was separated selectively from by-product organic acids,residual substrate and divalent ion in simulated fermentation broth by the same nanofiltration membrane.
Many impurities existed in succinic acid fermentation broth,such as by-product organic acids(formic acid,acetic acid),residual substrate(glucose) and divalent ion(Mg2+,SO42-),which was unfavorable to purification.Separation of succinic acid from simulated fermentation broth by nanofiltration membrane LNG-NF-016(MWCO 160) was studied,For this purpose,the effect of operating parameters such as pH in the feed solution,operation pressure,MgSO4 concentration,succinic acid concentration,glucose concentration,cross-flow rate and operation temperature on nanofiltration was investigated using an experimental design technique.The selectivity of nanofiltration depended on the degree of dissociation of succinic acid.At pH about 3.0,undissociated succinic acid could be separated from glucose,and MgSO4with succinic acid permeation of 98% and glucose and MgSO4 with rejection more than 85%.The divalent succinate could be separated from acetate and formate at pH about 6.0,succinic acid rejection of 90%,succinic acid concentration about 121 g/L in retentate,formic acid and acetic acid concentrations below 5 g/L.Succinic acid was separated selectively from by-product organic acids,residual substrate and divalent ion in simulated fermentation broth by the same nanofiltration membrane.
引文
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[2]詹晓北,朱一晖.琥珀酸发酵生产工艺及其产品市场[J].食品科技,2003,(2):44-49.
[3]吴昊,姚忠,姜岷,等.丁二酸发酵液的膜分离过程研究[J].食品与发酵工业,2007,33(5):42-46.
[4]Bouchoux A,Balmann H R,Lutin F.Nanofiltration ofglucose and sodium lactate solutions Variations ofretention between single-and mixed-solute solutions[J].JMembr Sci,2005,258:123-132.
[5]Han I S,Cheryan M.Nanofiltration of model acetate so-lutions[J].J Membr Sci,1995,107:107-113.
[6]Choil J H,Fukushi K,Yamamoto K.A study on the re-moval of organic acids from wastewaters using nanofil-tration membranes[J].Sep Purif Technol,2007,59(1):17-25.
[7]Moresi M,Ceccantoni B,Presti S L.Modelling of am-monium fumarate recovery from model solutions bynanofiltration and reverse osmosis[J].J Membr Sci,2002,209:405-420.
[8]Kang S H,Chang Y K,Chang H N.Recovery of Am-monium Lactate and Removal of Hardness from Fer-mentation Broth by Nanofiltration[J].Biotechnology,Progress,2004,20:764-770.
[9]陈可泉,韦萍,蔡婷,等.反相高效液相色谱在发酵制备丁二酸中的应用[J].生物加工过程,2005,3(2):50-52.
[10]时红,孙新忠,范建华,等.水质分析方法与技术[M].地震出版社,北京:2001:140-195.
[11]王晓琳,丁宁.反渗透和纳滤技术与应用[M].化学工业出版社,北京:2005:20-49.
[12]Kang S H,Chang Y K.Removal of organic acid saltsfrom simulated fermentation broth containing succinateby nanofiltration[J].J Membr Sci,2005,246:49-57.
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