Based on the methods of field geological survey, ultramicrostructural and microstructural observation in a laboratory and petrofabric analysis of tectonites, two ductile shear zones trending in NWW and NE in the Jianchaling area are determined. The former occurs in the northern parts of the study area while the latter in the southern area and they intersect each other in Jianchaling with a gentle wave-like shape in both strikes and dips. The macroscopical, ultramicrostructural and microstructural characteristics of the ductile shear zones prove that the two ductile shear zones are of conjugation, and experienced at least three periods. The ductile shear zones play a very important role in controlling gold and nickel mineralization in the study area.