The data of auto-monitoring system is used to discuss and analyze the regime of karst groundwater and the contaminate features of water quality in Jila Subterranean River mouth. The results show that there is 24 hour change period in water quality, which lags for 12 hours behind the resident domestic water discharge. Whereas, the change in concentrations of Cl- and ammonia nitrogen is of 6 hours phase difference, which proves that the origin of Cl- not only comes from domestic sewage but also intermittent industrial waste water discharge. The automatic monitoring to water quality can provide decision-making supports to water environment management and water resources application, and play more important role on karst water resources evaluation in Southwest China Karst Area.