Based on a series of analytical data, this paper studied the isotopic geochemical characteristics of the Lengshuibeigou lead-zinc-silver ore deposit in Luanchuan County, Henan Province, so as to probe into the variation regularity of elements in the East Qinling lead-zinc-silver metallogenic system. The ore-forming material source, the composition of ore-forming hydrothermal fluids and the metallogenic environment of the ore deposit were analyzed. 40Ar-39Ar dating of quartz was made, and the result shows that the silver-lead-zinc bearing quartz veins are products of Late Yanshanian metallogenic process. The achievements obtained are valuable in ore-prospecting practice.