Substrate specificity of Mortierella alpina Δ9-III fatty acid desaturase and its value for the production of omega-9 MUFA
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- 作者：Guangfei Hao ; Haiqin Chen ; Bo Yang ; Kai Du ; Hongchao Wang ; Zhennan Gu ; Hao Zhang ; Wei Chen and Yong Q. Chen
- 关键词：&Delta ; 9-III Fatty acid desaturase ; Mortierella alpina ; Overexpression ; Substrate specificity
- 刊名：European Journal of Lipid Science and Technology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：118
- 期：5
- 页码：753-760
- 全文大小：262K
- ISSN：1438-9312
文摘
The fatty acid desaturase (FADS9-III) gene from M. alpina ATCC 32222 was cloned and overexpressed in a homologous system. Lipid analysis via GC-MS revealed that the Δ9-III fatty acid desaturase had moderate activity with palmitic acid (C16:0) and stearic acid (C18:0) as substrates. Palmitoleic acid (C16:1) and oleic acid (C18:1) contents increased 4.0- and 1.94-fold in the FADS9-III overexpression clones compared to the wild-type strain. Low temperature (12°C) significantly improved the conversion ratio of C16:0 to C16:1 (9.12-fold increase). In addition, the FADS9-III fatty acid desaturase had specific activity at the ω9 position with lignoceric acid (C24:0) as a substrate.