Injured hepatocyte-released microvesicles induce bone marrow-derived mononuclear cells differentiation
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- 作者：Laura Simona ; b ; Mó ; nica Ló ; peza ; b ; Carolina Uribe-Cruza ; Davi Fernandes Peralvo Vergaraa ; Lucia Sillac ; Ursula Mattea ; b ; d ; umatte@hcpa.ufrgs.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：BMMCs ; Bone marrow-derived mononuclear cells ; CCl4 ; Carbon tetrachloride ; NAD ; Non-adherent ; MVs ; Microvesicles ; MSCs ; Mesenchymal stem cells ; ED1+ ; Monocyte/macrophage marker ; MACS ; Magnetic-activated cell sorting ; DMEM ; Dulbecco's modified eagle medium ; FBS ; Fetal bovine serum ; P/S ; Penicillin/streptomycin ; CO2 ; Carbon dioxide ; FITC ; Fluorescein isothiocyanate ; PE ; Phycoerythrin ; CD29 ; Integrin β1 ; CD90 ; Thy-1 ; CD45 ; lymphocyte common antigen ; CD31 ; Platelet endothelial cell adhesion molecule
- 刊名：Differentiation
- 出版年：2015
- 出版时间：July-October 2015
- 年：2015
- 卷：90
- 期：1-3
- 页码：40-47
- 全文大小：2618 K
文摘
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Differentiation of NAD BMMC into hepatocyte-like cells, other than MSC and monocytes.
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Expression of hepatocyte genes and proteins occurred after 24 h of co-culture.
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Only co-culture with injured cells promoted NAD BMMCs differentiation.
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Isolated MVs from co-culture with injured hepatocytes carried liver-specific mRNAs.
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The uptake of MVs from NAD cells was confirmed in Albumin and PKH26-positive cells.