Propagation of field HP-PRRSV in MARC-145 is cell-density dependent and the propagation is more effective in overgrown cells.
Apoptosis triggered automatically in the overgrown MARC-145 cells promotes the propagation of field virus in these cells.
Induction of apoptosis in MARC-145 cells mimics the promotion and further increased viral production.
A vigorous viral RNA replication and fast virus release in early life cycle of viral infection were detected in the apoptosis induced cells.
Propagation of wild type PRRSV in apoptosis induced cells may provide a way to replicate the virus for clinical diagnosis and vaccine development.