Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating
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- 作者：Doreen Matthies1 ; Olivier Dalmas2 ; Mario J. Borgnia1 ; Pawel K. Dominik2 ; Alan Merk1 ; Prashant Rao1 ; Bharat G. Reddy2 ; Shahidul Islam2 ; Alberto Bartesaghi1 ; Eduardo Perozo2 ; eperozo@uchicago.edu" class="auth_mail" title="E-mail the corresponding author ; Sriram Subramaniam1 ; ss1@nih.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：asymmetry ; conformational change ; direct electron detector ; ion channel ; membrane protein structure ; single-particle cryo-electron microscopy
- 刊名：Cell
- 出版年：2016
- 出版时间：11 February 2016
- 年：2016
- 卷：164
- 期：4
- 页码：747-756
- 全文大小：4109 K
文摘
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Find 3.8 Å resolution cryo-EM structure of the ∼200 kDa magnesium channel CorA
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Mg2+-free CorA exhibits dramatic loss of symmetry in the cytoplasmic domain
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Identify two discrete, asymmetric conformations of Mg2+-free CorA
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Inter-subunit Mg2+ is important for stabilizing the closed state of CorA