Application of a new model based on oxygen balance to determine the oxygen uptake rate in mammalian cell chemostat cultures
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- 作者：Yandi Rigual-Gonzá ; leza ; b ; Lucí ; a Gó ; meza ; Jonathan Nú ; ñ ; eza ; Mauricio Vergaraa ; Alvaro Dí ; az-Barreraa ; Julio Berriosa ; Claudia Altamiranoa ; c ; claudia.altamirano@pucv.cl" class="auth_mail" title="E-mail the corresponding author
- 关键词：(s) and (b) subscripts ; Denote the medium-headspace air and medium-bubbles interfaces ; respectively ; CL ; Dissolved oxygen concentration in the culture medium ; CL0 ; Dissolved oxygen concentration in the feed ; F ; Volumetric flow of culture medium in the feed ; D ; Dilution rate ; C* ; Equilibrium concentration of oxygen in the culture medium ; kLa ; Volumetric oxygen transfer coefficient in the liquid phase ; qO2 ; Specific oxygen uptake rate ; T ; Dead time of oxygen probe ; E(t) ; Signal sent by the oxygen
- 刊名：Chemical Engineering Science
- 出版年：2016
- 出版时间：2 October 2016
- 年：2016
- 卷：152
- 期：Complete
- 页码：586-590
- 全文大小：641 K
文摘
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Use of a model based on total O2 balance to estimate the OUR in mammalian cells.
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The estimation of the OUR is more accurate when the proposed model is used.
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OUR and qO2 values differ depending on the model used.
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In chemostat at 33 °C, O2 transfer through the headspace must be considered.