In vitro grafting of cashew (Anacardium occidentale L.)

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• 作者：Thimmappaiah ; Puthra ; G.T. ; Anil ; Shirly Raichal
• 关键词：Micrografting ; Cashew ; Rejuvenation ; Micropropagation ; In vitro
• 刊名：Scientia Horticulturae
• 出版年：2002
• 出版时间：January 31, 2002
• 年：2002
• 卷：92
• 期：2
• 页码：177-182
• 全文大小：92.5 K

文摘

A successful micrografting technique in cashew was developed using in vitro germinated seedlings as rootstocks and axenic shoot cultures (shoot-tip and nodal cultures) established from mature tree source as microscions. In vitro germinated seedlings, which emerged 20–25 days after inoculation on absorbent cotton, were decapitated and used as rootstock. Mature tree explants initiated on hormone-free Murashige and Skoog [Physiol. Plant. 15 (1962) 473] (MS) modified medium were made into scion of 3–15mm length for grafting. Micrografts could be easily cultured on hormone-free liquid half-MS medium and were potted out after 10–12 weeks of culture growth. Grafting success was dependent on the method of grafting and size of the scion. Shoot-tip grafting and side grafting were equally successful (79.5–100 % ). Length of scion shoot had significant effect on micrografting success. Graft success was high (79.5 % ) when the scion length was >5mm and it was less (0.5 % ) when size of scion was small (3–5mm). Scion presoaked in either water or 0.01 % ascorbic acid and 0.015 % citric acid (1:1) reduced phenolic browning and drying of scion. Micrografting techniques standardized could be used for rejuvenation of shoot explants of mature tree.