Mapping and identification of cassava mosaic geminivirus DNA-A and DNA-B genome sequences for efficient siRNA expression and RNAi based virus resistance by transient agro-infiltration studies
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- 作者：Basavaprabhu L. Patila ; b ; blpatil2046@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Basavaraj Bagewadib ; 1 ; Jitender S. Yadavb ; 2 ; Claude M. Fauquetb ; 3
- 关键词：Geminivirus ; RNA interference ; Virus resistance ; Transient studies
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 February 2016
- 年：2016
- 卷：213
- 期：Complete
- 页码：109-115
- 全文大小：1415 K
文摘
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Maximum siRNA accumulated from 3 to 7 day after agroinfiltration of RNAi construct.
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A gap of 3 days between agro-infiltration and virus inoculation was critical.
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Maximum resistance was obtained by targeting AC2 ORF, in addition to AC1 and AC4.
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In the ACMV DNA-B component targeting BC1 gave the best results.
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The 5′-half of AC3 and AC4 ORFs yielded more siRNA than the corresponding 3′-half.