GATA-4 synergizes with PKG-1α to activate NPPA promoter in response to cGMP.
S261 of GATA4 is the site for PKG-1α phosphorylation.
GATA4's DNA binding activity is enhanced by PKG-1α via both phosphorylation and physical association.
GATA4 is preferentially activated by PKG-1α, but not by PKA on NAAP promoter.
Human disease-linked GATA4 mutations suppress PKG-1α-dependent S261 phosphorylation of GATA4.