Comparing strategies for covalent immobilization of antibody (Ab) for immunosensor.
Asn–AuNP better candidate than EDC–NHS activated cit-AuNP for immobilizing Ab.
Orthogonal immobilization of Ab with 88% active Ab for Asn–AuNP-Ab immunosenor.
Asn–AuNP–Ab immunosensor sensitivity 2.5 times that of cit-AuNP–EDC–NHS–Ab.
Immunosensor dynamic range: Asn–AuNP–Ab>cit-AuNP–Ab=cit-AuNP–EDC–NHS–Ab.