Data set for describing the elaboration of a compatible Gateway-based co-expression vector set and supporting its validation

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• 作者：Loubna Salim ; Claire Feger ; Didier Busso ; ^{didier.busso@cea.fr}
• 关键词：Co-expression ; Expression screening ; Gateway ; Protein complex ; Recombinational cloning ; Versatility
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：Complete
• 页码：946-955
• 全文大小：2369 K
• 卷排序：9

文摘

This article contains Supplementary Data including methods and figures that relate to the article entitled “Construction of a compatible Gateway-based co-expression vector set for expressing multiprotein complexes in E. coli” (L. Salim, C. Feger, D. Busso, 2016) [1] that describes the elaboration and the validation of a set of versatile compatible plasmids for co-expression studies in Escherichia coli.Here, we describe experimental procedures for plasmid construction and recombinant protein expression. We give the list of the 33 (co)-expression plasmids encoding fluorescent protein and we show extensive experimental data obtained for all combinations tested for validating our vector set.