Silk fibroin nanoparticles: Efficient vehicles for the natural antioxidant quercetin

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• 作者：Antonio Abel Lozano-Pé ; rez^a ; ^{antonioa.lozano@carm.es} ; Hé ; ctor Correa Rivero^b ; Marí ; a del Carmen Pé ; rez Hern&aacute ; ndez^c ; Ana Pag&aacute ; n^a ; Mercedes G. Montalb&aacute ; n^d ; Gloria Ví ; llora^d ; José ; Luis Cé ; nis^a
• 关键词：Quercetin ; Silk fibroin ; Nanoparticles ; Polyphenols ; Antioxidant activity ; Drug delivery
• 刊名：International Journal of Pharmaceutics
• 出版年：2017
• 出版时间：25 February 2017
• 年：2017
• 卷：518
• 期：1-2
• 页码：11-19
• 全文大小：1877 K
• 卷排序：518

文摘

This article describes how silk fibroin nanoparticles (SFNs) are capable of adsorbing and releasing quercetin (Q) and how its integrity is highly preserved, as confirmed by antioxidant activity assays. Q loading onto SFNs was optimized in terms of the Q/SFN ratio (w/w), time of adsorption and solvent mixture. Quercetin-loaded silk fibroin nanoparticles (QSFNs) were characterized using the dynamic light scattering technique to measure the diameter (Z-Average) and Z-potential (ζ). Loaded particles were slightly bigger than the SFNs, while their ζ was less negative. The antioxidant activity against DPPH showed that the Q loaded in QSFNs not only retains the antioxidant activity but also has a synergistic scavenging activity due the intrinsic antioxidant activity of the SF. The drug loading content (DLC) and the encapsulation efficiency (EE) varied with the relation between Q and SFN in the loading solution. The sustained release of Q occurred throughout the experiment both in phosphate buffer saline (pH 7.4) and simulated intestinal fluid (pH 6.8). The results point to SFNs as promising candidates for Q loading, transport and gastrointestinal delivery with potential applications in nanomedicine, while retaining their nano-size and their antioxidant properties.