Kinetic isotope effect for human 5-lipoxygenase is reported.
Hydrogen abstraction is the rate-liming step for human 5-lipoxygenase.
Change in regioselectivity is observed upon deuteration at C-7 of arachidonic acid.
The viscosity experiments influence the Hkcat more than Dkcat and not the overall kcat/KM for AA and 7,7-d2-AA respectively.
Enzyme–substrate dynamics is proposed to affect the catalysis.