A novel medium for the production of cephamycin C by Nocardia lactamdurans
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- 作者：L.D. Kagliwal ; S.A. Survase ; R.S. Singhal
- 关键词：Cephamycin C ; Nocardia lactamdurans ; Solid-state fermentation ; 1 ; 3-Diaminopropane
- 刊名：Bioresource Technology
- 出版年：2009
- 出版时间：May 2009
- 年：2009
- 卷：100
- 期：9
- 页码：2600-2606
- 全文大小：840 K
文摘
In this study, Nocardia lactamdurans NRRL 3802 was explored for the first time for production of cephamycin C by using solid-state fermentation. The effects of various substrates, moisture content, inoculum size, initial pH of culture medium, additional nitrogen source and amino acids were investigated for the maximum production of cephamycin C by N. lactamdurans NRRL 3802 in solid-state fermentation. Subsequently, selected fermentation parameters were further optimized by response surface methodology (RSM). The soybean flour as a substrate with moisture content of 65 % , initial pH of culture medium of 6.5 and inoculum size of 109 CFU/ml (2 × 108 CFU/gds) at 28 ± 2 °C after 4 days gave maximum production of 15.75 ± 0.27 mg/gds of cephamycin C as compared to 8.37 ± 0.23 mg/gds before optimization. Effect of 1,3-diaminopropane on cephamycin C production was further studied, which further increased the yield to 27.64 ± 0.33 mg/gds.