A Comparative Transcriptome and Proteome Analysis in Rat Models Reveals Effects of Aging and Diabetes on Expression of Neuronal Genes

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• 作者：Qian Xu¹ ; ^&dagger ; ; Jing Cai¹ ; ^&dagger ; ; Yi-Bo Cong¹ ; Shao-Jian Xiao² ; Yun Liu¹ ; Wei Qin¹ ; Shi-Ya Chen¹ ; Hong Shi¹ ; ^{shihong3327@sina.com}
• 关键词：diabetes ; aging ; neuronal genes ; transcriptome ; proteome
• 刊名：International Journal of Gerontology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：10
• 期：4
• 页码：212-217
• 全文大小：2031 K
• 卷排序：10

文摘

To understand neuronal molecular changes in senile diabetes we established a rat senile diabetes model and analyzed transcriptome and proteome changes.MethodsWistar rats were fed a high sugar, high fat diet for 16 months to induce diabetes. Non-diabetic aged rats and young rats were used as controls. Transcript and protein levels in the liver were then analyzed by microarray and antibody arrays, respectively.ResultsNeuronal genes that were differentially expressed between senile diabetic rats, non-diabetic aged rats, and young rats were distributed across 12 pathways and 23 Gene Ontology (GO) clusters. Among them, 2267 genes were aging-related, 1230 genes were diabetes-associated, and 9 proteins might be associated with neurological disorders.ConclusionIn this study, we investigated transcriptome and proteome changes in animal models, analyzed the impact of aging and diabetes on neuronal molecules, and confirmed the correlations. Our study provides support for further studies on mechanisms of neuronal diseases.