Highly efficient and low-cost method to isolate human blood monocytes with high purity
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- 作者：Fabian Stö ; gbauer ; Markus Neumeier ; Johanna Weigert ; Josef Wanninger ; Margot Gr ; l ; Karla Lehle ; Gerd Schmitz ; Charalampos Aslanidis ; Andreas Schä ; ffler ; Jü ; rgen Schö ; lmerich ; Christa B
- 关键词：Blood monocytes ; CD14 MicroBeads ; Purity
- 刊名：Journal of Immunological Methods
- 出版年：2008
- 出版时间：20 August 2008
- 年：2008
- 卷：337
- 期：1
- 页码：78-80
- 全文大小：264 K
文摘
Several techniques are available to purify circulating blood monocytes for research. CD14-containing MicroBeads are suitable and reliable tools to reproducibly isolate human monocytes with a high purity but are quite expensive. This report describes that a comparable number of highly pure monocytes can be isolated from samples using up to tenfold lower amounts of CD14-MicroBeads. MicroBeads are widely used to isolate different cell populations and with this report more researchers may be encouraged to use this highly efficient, low-cost and thus affordable method to pursue their scientific goals.