Cystamine ameliorates ventricular hypertrophy associated with modulation of IL-6-mediated signaling in lupus-prone mice

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• 作者：Bor-Show Tzang^a ; ^b ; ^c ; Tsai-Ching Hsu^b ; ^d ; Tzy-Yen Chen^e ; ^f ; Chih-Yang Huang^g ; ^h ; ⁱ ; Sin-Lun Li^a ; Shao-Hsuan Kao^a ; ^b ; ^{kaosh@csmu.edu.tw}
• 关键词：Cystamine ; Lupus-prone mice ; Left ventricular hypertrophy ; IL-6 ; MEK5
• 刊名：Life Sciences
• 出版年：2013
• 出版时间：9 April, 2013
• 年：2013
• 卷：92
• 期：12
• 页码：719-726
• 全文大小：1280 K

文摘

Aims

The aim of this study is to investigate the protective effects of cystamine on lupus-associated cardiac hypertrophy.

Main methods

Balb/c and lupus-prone NZB/W-F1 mice were individually randomized into sham group (saline, n = 16) and cystamine group (n = 16). Mice received saline or cystamine (100 mmol in 100 ¦ÌL saline) by daily intraperitoneal injection for 2 consecutive weeks. Morphological, histological, and biochemical alterations were investigated.

Key findings

Cystamine decreased both left ventricular (LV) mass and LV mass/tissue-to-blood ratio (TBR) in NZB/W-F1 mice (p < 0.05), whereas slight effects were observed in Balb/c mice. Moreover, cystamine reduced levels of atrial natriuretic peptide (ANP), C-reactive protein (CRP), heart type-fatty acid binding protein (h-FABP), creatine kinase-MB (CK-MB) and IL-6 in LV tissues of NZB/W-F1 mice (p < 0.05). Additionally, in LV tissues of NZB/W-F1 mice, suppression of hypertrophic signaling mediated by IL-6 in response to administration of cystamine was revealed, including phosphorylation of MEK5, ERK5, c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38) (p < 0.05).

Significance

Cystamine alleviated LV hypertrophy in NZB/W-F1 mice as a result of decrease in hypertrophic mediators and suppression of IL-6 mediated hypertrophic signaling.