Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity

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• 作者：Ochuko L. Erukainure^a ; ^b ; ^c ; ^{loreks@yahoo.co.uk" class="auth_mail" title="E-mail the corresponding author} ; Ahmed M. Mesaik^b ; ^d ; Aliyu Muhammad^b ; ^e ; Chika I. Chukwuma^c ; Neha Manhas^f ; Parvesh Singh^f ; Oluwole S. Aremu^f ; Md. Shahidul Islam^c
• 关键词：Antiinflamation ; Antioxidant ; Immunomodulation ; Oxidative burst
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：83
• 期：Complete
• 页码：1478-1484
• 全文大小：1461 K

文摘

The immunomodulatory potentials of the crude methanolic extract and fractions [n-hexane (Hex), n-dichloromethane (DCM), ethyl acetate (EtOAc) and n–butanol (BuOH)] of Clerodendrum volubile flowers were investigated on whole blood phagocytic oxidative burst using luminol-amplified chemiluminescence technique. They were also investigated for their free radicals scavenging activities. The DCM fraction showed significant (p < 0.05) anti-oxidative burst and free radical scavenging activities indicating high immunomodulatory and antioxidant potencies respectively. Cytotoxicity assay of the DCM fraction revealed a cytotoxic effect on CC-1 normal cell line. GCMS analysis revealed the presence of triacetin; 3,6-dimethyl-3-octanol; 2R – Acetoxymethyl-1,3,3–trimethtyl – 4 t – (3-methyl-2-buten-1-yl) – 1c – cyclohexanol and Stigmastan – 3,5–diene in DCM fraction. These compounds were docked with the active sites of cyclooxygenase-2 (COX-2). Triacetin, 3,6-dimethyl-3-Octanol, and 2R-Acetoxymethyl-1,3,3-trimethtyl-4t-(3-methyl-2-buten-1-yl)-1c-cyclohexanol docked comfortably with COX-2 with good scoring function (-CDocker energy) indicating their inhibitory potency against COX-2. 3,6–dimethyl-3-Octanol, displayed the lowest predicted free energy of binding (−21.4 kcal mol⁻¹) suggesting its stronger interaction with COX-2, this was followed by 2R – Acetoxymethyl-1, 3, 3-trimethtyl-4t-(3-methyl-2-buten-1-yl)-1c-cyclhexanol (BE = −20.5 kcal mol⁻¹), and triacetin (BE = −10.9 kcal mol⁻¹). Stigmastan – 3,5–diene failed to dock with COX-2. The observed suppressive effect of the DCM fraction of C. volubile flower methanolic extract on phagocytic oxidative burst indicates an immunomodulatory potential. This is further reflected in its free scavenging activities and synergetic modulation of COX-2 activities by its identified compounds in silico.