Expression of a novel bacteriocin—the plantaricin Pln1—in Escherichia coli and its functional analysis
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- 作者：Fanqiang Meng mfq2012@outlook.com" class="auth_mail" title="E-mail the corresponding author ; Haizhen Zhao zhaohz@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Chong Zhang zhangchong@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Fengxia Lu lufengxia@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaomei Bie bxm43@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhaoxin Lu ; fmb@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Plantaricin ; Heterologous expression ; Antimicrobial activity ; MRSE
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：119
- 期：Complete
- 页码：85-93
- 全文大小：1744 K
文摘
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A novel bacteriocin gene, pln1, was identified using whole-genome sequencing data.
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The putative bacteriocin was successfully overexpressed in Escherichia coli.
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A yield of 9–11 mg/l was obtained for PLN1.
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Antimicrobial activity of PLN1 and its value as a food biopreservative were assessed.
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PLN1 had antimicrobial activity against some gram-positive and lactic acid bacteria.