Validation of putative reference genes for gene expression studies in heat stressed and α-MSH treated melanocyte cells of Bos indicus using real-time quantitative PCR
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- 作者：Renuka Choudharya ; c ; rc_07@live.in" class="auth_mail" title="E-mail the corresponding author ; Sudarshan Kumarb ; kumarsudershan@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Sohan V. Singha ; sohanvir2011@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Anil K. Sharmac ; anilbiotech18@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Talla Sridhar Gouda ; tallasridhargoud@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Amrendra K. Srivastavaa ; amar00007@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Anil Kumara ; anilbiotech84@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ashok K. Mohantyb ; ashokmohanty1@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ramesh C. Upadhyaya ; upadhyay.ramesh@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bos indicus ; Heat stress ; Internal reference genes ; Melanocyte cells ; geNorm ; NormFinder ; BestKeeper
- 刊名：Molecular and Cellular Probes
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：30
- 期：3
- 页码：161-167
- 全文大小：701 K
文摘
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15 internal reference genes (IRGs) were tested that could serve as best IRG in melanocyte cells.
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The melanocyte cells were isolated and cultured in-vitro from Bos indicus (Tharparkar cattle).
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geNorm, Normfinder and Bestkeeper algorithms were used to measure the gene transcript stability.
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The present study reveals UXT, HPRT and RPS23 as the best internal reference genes in melanocyte cells.