A transthylakoid proton gradient and inhibitors induce a non-photochemical fluorescence quenching in unicellular algae Nannochloropsis sp.

详细信息    查看全文

• 作者：Shaona Cao^a ; ^b ; Xiaowen Zhang^b ; Dong Xu^b ; Xiao Fan^b ; Shanli Mou^b ; Yitao Wang^a ; Naihao Ye^b ; ^{yenh@ysfri.ac.cn} ; Wenqi Wang^a ; ^{wenqiwang@qau.edu.cn}
• 关键词：Ax ; antheraxanthin ; Chl ; chlorophyll ; DCCD ; N ; N&prime ; -dicyclohexyl-carbodiimide ; DCMU ; 3-(3 ; 4-dichlorophenyl)-1 ; 1-dimethylurea ; DC ; diadinoxanthin cycle ; Ddx ; diadinoxanthin ; DTT ; dithiothreitol ; Dtx ; diatoxanthin ; NPQ ; non-photochemical fuorescence quen
• 刊名：FEBS Letters
• 出版年：2013
• 出版时间：2 May, 2013
• 年：2013
• 卷：587
• 期：9
• 页码：1310-1315
• 全文大小：693 K

文摘

Non-photochemical quenching (NPQ) of chlorophyll fluorescence is thought to be an indicator of an essential regulation and photoprotection mechanism against high-light stress in photosynthetic organisms. In this report, special chemicals were used to perturb the kinetics of the ¦¤pH build-up and the xanthophyll cycle (XC) in Nannochloropsis sp. We found that NPQ was stimulated rapidly on exposure to high light and relaxed rapidly in darkness. The ¦¤pH could be obligatory for NPQ and ¦¤pH alone was not sufficient to induce NPQ. The XC, being strictly mediated by ¦¤pH, was also essential for NPQ. The results demonstrate that the mechanism of NPQ in Nannochloropsis sp. resembled that of diatoms.