Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid - Monoacylated phosphatidylinositol dimannoside
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- 作者：Zuzana Svetlí ; ková ; a ; Peter Bará ; thb ; Mary Jacksonc ; Jana Kordulá ; ková ; a ; Katarí ; na Miku&scaron ; ová ; a ; mikusova@fns.uniba.sk" class="auth_mail
- 关键词：Mycobacterium tuberculosis ; Mannosylated glycoconjugates ; Expression system
- 刊名：Protein Expression and Purification
- 出版年：August 2014
- 年：2014
- 卷：100
- 期：Complete
- 页码：33-39
- 全文大小：1322 K
文摘
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We used mycobacterial pJAM2 expression system to obtain acyltransferase MSMEG_2934.
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MSMEG_2934 protein purified to homogeneity was intact and active.
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MSMEG_2934 attaches palmitoyl group to phosphatidylinositol dimannoside.