Heme-induced contractile dysfunction in Human cardiomyocytes caused by oxidant damage to thick filament proteins
详细信息 查看全文
- 作者：Gerardo Alvaradoa ; b ; Viktó ; ria Jeneyb ; c ; Attila Tó ; tha ; É ; va Csőszd ; Gergő Kalló ; d ; An T. Huynha ; Csaba Hajnala ; Judit Kalá ; sza ; Enikő T. Pá ; sztora ; Istvá ; n É ; desa ; Magnus Grame ; Bo Akerströ ; me ; Ann Smithf ; John W. Eatong ; Gyö ; rgy Ballac ; h ; Zoltá ; n Pappa ; Jó ; zsef Ballab ; c ; balla@internal.med.unideb.hu" class="auth_mail" title="E-mail the corresponding author
- 关键词：A1M ; Alpha-1-microglobulin ; BSA ; Bovine serum albumin ; cMyBPC ; Cardiac myosin binding protein C ; CPK ; Creatinine phosphokinase ; DMF ; Dimethylformamide ; DTDP ; 2 ; 2&rsquo ; -Dithiodipyridine ; DTT ; Dithiothreitol ; ECL ; Enhanced chemiluminescence ; EGTA ; Ethylene glycol tetra-acetic acid ; F ; Isometric force ; Fo ; Force at saturating [Ca2+] ; Fpassive ; Passive force ; Ftotal ; Total peak isometric force ; HO-1 ; Heme oxygenase 1 ; IC50 ; Half maximal inhibitory concentration ; LDH ; Lactate dehydrogenase ; MHC ; Myo
- 刊名：Free Radical Biology and Medicine
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：89
- 期：Complete
- 页码：248-262
- 全文大小：1623 K
文摘
- •
Binding of heme to MLC-1 initiate.
- •
Five fold increase in Ca2+-independent passive force and decrease in Ca2+-regulated maximal activity by 90% permeabilized in human cardiomyocytes.
- •
Heme-mediated SH oxidation of titin, α-actinin, filamin C, myosin heavy chain, and cardiac myosin binding protein C contributing to deterioration of cardiac force generation.
- •
Heme-evoked stable sulfenic acid formation in myosin heavy chain, cardiac myosin binding protein C, and α-actinin, which was not reversed by DTT, probably contributing to the physiological effects.
- •
Binding of heme to hemopexin or alpha-1-microglobulin prevented its effects on cardiomyocyte contractility.