Inhibition of Mayaro virus infection by bovine lactoferrin

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• 作者：Carlos A.M. Carvalho^a ; Ivanildo P. Sousa Jr.^b ; Jerson L. Silva^a ; André ; a C. Oliveira^a ; Rafael B. Gonç ; alves^c ; Andre M.O. Gomes^a ; ^{amog@bioqmed.ufrj.br" class="auth_mail}
• 关键词：MAYV ; Mayaro virus ; bLf ; bovine lactoferrin ; GAG ; glycosaminoglycan ; DMEM ; Dulbecco壮s modified Eagle壮s medium ; MOI ; multiplicity of infection ; PBS ; phosphate-buffered saline ; SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; DiD ; 1 ; 1壮-dioctadecyl-3 ; 3 ; 3壮 ; 3壮-tetramethylindodicarbocyanine ; 4-chlorobenzenesulfonate salt ; FITC ; fluorescein-5-isothiocyanate ; MTT ; 3-(4 ; 5-dimethylthiazolyl-2-yl)-2 ; 5-diphenyltetrazolium bromide ; PFU ; plaque-forming unit
• 刊名：Virology
• 出版年：March, 2014
• 年：2014
• 卷：452-453
• 期：Complete
• 页码：297-302
• 全文大小：1893 K

文摘

Mayaro virus (MAYV) is an arbovirus linked to several sporadic outbreaks of a highly debilitating febrile illness in many regions of South America. MAYV is on the verge of urbanization from the Amazon region and no effective antiviral intervention is available against human infections. Our aim was to investigate whether bovine lactoferrin (bLf), an iron-binding glycoprotein, could hinder MAYV infection. We show that bLf promotes a strong inhibition of virus infection with no cytotoxic effects. Monitoring the effect of bLf on different stages of infection, we observed that virus entry into the cell is the heavily compromised event. Moreover, we found that binding of bLf to the cell is highly dependent on the sulfation of glycosaminoglycans, suggesting that bLf impairs virus entry by blocking these molecules. Our findings highlight the antiviral potential of bLf and reveal an effective strategy against one of the major emerging human pathogens in the neotropics.