Stable and enhanced gene expression in Clostridium acetobutylicum using synthetic untranslated regions with a stem-loop
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- 作者：Joungmin Leea ; b ; 1 ; Yu-Sin Janga ; b ; 2 ; Eleftherios T. Papoutsakisc ; Sang Yup Leea ; b ; leesy@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Clostridium acetobutylicum ; Gene overexpression ; 5&prime ; untranslated region
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 July 2016
- 年：2016
- 卷：230
- 期：Complete
- 页码：40-43
- 全文大小：644 K
文摘
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Short single-stranded UTR decreased gene expression in Clostridium acetobutylicum.
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Addition of a stem-loop at the 5′ end of mRNA increased mRNA level.
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Addition of a stem-loop at the 5′ end of mRNA increased protein expression level.