文摘
Edwardsiella tarda is a gram-negative bacterial pathogen of fish and animals. A number of gram-negative bacteria have quorum-sensing systems and produce N-acylhomoserine lactone (AHL) that they use as a quorum-sensing signaling molecule. We have already reported that E. tarda NUF251 produces AHLs and has the AHL-synthase gene, edwI. Inactivation of NUF251 edwI induces expression of an approximately 45?kDa extracellular protein, identified as a flagellin encoded by FliC. Mutation of edwI also changes the motility pattern of NUF251 from a radial expansion pattern to concentric rings. The addition of exogenous AHL was capable of restoring normal motility to NUF251 edwI mutants. These results demonstrate that quorum sensing negatively regulates motility and expression of the FliC protein.