Molecular identification and characterization of peptide: N-glycanase from Schizosaccharomyces pombe

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• 作者：Fengxue Xin ; Shengjun Wang ; Lei Song ; Quanfeng Liang ; Qingsheng Qi
• 关键词：Schizosaccharomyces pombe ; Glycanase ; Deglycosylation ; Evolution ; Glycoprotein ; Protein degradation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：18 April 2008
• 年：2008
• 卷：368
• 期：4
• 页码：907-912
• 全文大小：551 K

文摘

Peptide:N-glycanase (PNGase) is an enzyme responsible for deglycosylation of misfolded glycoproteins in so-called endoplasmic reticulum-associated degradation (ERAD) system. In this study, we reported the molecular identification and characterization of SpPNGase (Schizosaccharomyces pombe PNGase). Enzymatic analysis revealed that SpPNGase deglycosylated the misfolded glycoproteins and distinguished native and denatured high-mannose glycoproteins in vitro. The deglycosylation activity was lost with the addition of chelating agent EDTA and was not restored by re-addition of metal ions. By construction of deletion mutant, we confirmed that N-terminal -helix of SpPNGase was responsible for the protein–protein interaction. Combining the results from ternary structure prediction and dendrogram analysis, we suggested that the N-terminal -helices of PNGase are derived from evolutionary motif/peptide fusion.