A Drosophila melanogaster homologue of the human DEAD-box gene DDX1

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• 作者：Rafti ; Fiorella ; Scarvelis ; Dimitrios ; Lasko ; Paul F.
• 关键词：RNA-binding proteins ; cell transformation ; gene mapping ; helicases ; hnRNP U
• 刊名：Gene
• 出版年：1996
• 出版时间：June 1, 1996
• 年：1996
• 卷：171
• 期：2
• 页码：225-229
• 全文大小：703 K

文摘

DEAD-box genes are found throughout evolution and encode RNA-binding proteins. Such proteins include eukaryotic initiation factor-4A, which is essential for protein translation, Vasa, which is essential for germ line development, and a number of nuclear and mitochondrial RNA splicing factors. Transcription of a human DEAD-box gene, DDX1, is elevated in two retinoblastoma cell lines as a result of amplification of the immediate chromosomal region surrounding it, suggesting an important role for this gene in control of cell growth and division. We have isolated a Drosophila melanogaster (Dm) homologue (Ddx1) of DDX1 which is strikingly similar to the human gene. The similarity (58.3 % amino acid (aa) identity over 720 aa) extends beyond regions conserved in all DEAD-box proteins and covers the entire lengths of the proteins. The 2.7-kb Dm Ddx1 RNA is expressed throughout development, but its levels are elevated in early embryos. Ddx1 maps to polytene chromosome band 79D4 on the left arm of Dm chromosome 3.