Development of cathepsin-L cysteine proteinase based Dot-enzyme-linked immunosorbent assay for the diagnosis of Fasciola gigantica infection in buffaloes

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• 作者：Anju  ; Varghese ; O.K.  ; Raina  ; ;   ; ^{rainaok@rediffmail.com} ; Gaurav  ; Nagar ; Rajat  ; Garg ; P.S.  ; Banerjee ; B.R.  ; Maharana ; Justin D.  ; Kollannur
• 关键词：Fasciola gigantica ; Buffalo ; Cathepsin-L cysteine proteinase ; Dot-ELISA ; Immunodiagnosis
• 刊名：Veterinary Parasitology
• 出版年：2012
• 出版时间：10 February 2012
• 年：2012
• 卷：183
• 期：3-4
• 页码：382-385
• 全文大小：235 K

文摘

Native cathepsin-L cysteine proteinase (28 kDa) was purified from the excretory secretory products of Fasciola gigantica and was used for sero-diagnosis of F. gigantica infection in buffaloes by Dot-enzyme-linked immunosorbent assay (Dot-ELISA). The test detected F. gigantica field infection in these animals with a sensitivity of ?0 % . No specific IgG antibody binding was displayed by sera obtained from 76 buffaloes considered to be Fasciola and other parasite-free by microscopic examination of faeces and necropsy examination of liver, rumen and intestine. Additionally, sera from 156 Fasciola-free buffaloes, yet infected with Gigantocotyle explanatum, Paramphistomum epiclitum, Gastrothylax spp., Strongyloides papillosus and hydatid cyst were all negative, indicating that F. gigantica cathepsin-L cysteine proteinase does not cross-react with these helminth parasites in natural infection of the host. The data indicated that cathepsin-L cysteine proteinase based Dot-ELISA reached ?0 % sensitivity and 100 % specificity with relation to above parasites in the detection of bubaline fasciolosis. The present Dot-ELISA diagnostic assay is relevant to the field diagnosis of F. gigantica infection in buffaloes.