Human Gb3/CD77 synthase reveals specificity toward two or four different acceptors depending on amino acid at position 211, creating Pk, P1 and NOR blood group antigens
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- 作者：Radoslaw Kaczmareka ; radoslaw.kaczmarek@iitd.pan.wroc.pl" class="auth_mail" title="E-mail the corresponding author ; Maria Duka ; Katarzyna Szymczaka ; Elena Korchaginab ; Jolanta Tyborowskac ; Krzysztof Mikolajczyka ; Nicolai Bovinb ; Boguslaw Szewczykc ; Ewa Jaskiewiczd ; Marcin Czerwinskia
- 关键词：Enzyme promiscuity ; α1 ; 4-Galactosyltransferase ; P1 antigen ; Pk antigen ; Blood group antigens ; Glycosphingolipids ; Glycosyltransferase specificity
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：470
- 期：1
- 页码：168-174
- 全文大小：1015 K
文摘
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Gb3/CD77 synthase is the long-sought P1 synthase.
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The consensus Gb3/CD77 synthase is able to synthesize both Pk and P1 antigens.
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Gb3/CD77 synthase is another case of a promiscuous glycosyltransferase.
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p.Q211E substitution rouses the promiscuity, leading to synthesis of Pk, P1 and NOR.
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Acceptor specificity of Gb3/CD77 synthase is toggled by a single amino acid.