Establishment of a differentiated neuroglial primary cell culture system of the early postnatal rat arcuate nucleus (ARC).
Calcium imaging experiments indicate expression of P2X2 and P2Y1 purinoceptors in ARC neurons and/or astrocytes.
ARC neurons and astrocytes can be subdivided into partially temperature-dependent low and high desensitization groups.
Competitive binding assays with the radioligand [35S]dATPαS support functional purinoceptor expression in rat hypothalamus.