Development and current applications of assisted fertilization

详细信息    查看全文

• 作者：Gianpiero D. Palermo ; M.D. ; Ph.D. ; ^{gdpalerm@med.cornell.edu} ; Queenie V. Neri ; M.Sc. ; Devin Monahan ; B.Sc. ; Justin Kocent ; B.Sc. ; Zev Rosenwaks ; M.D.
• 关键词：ICSI ; spermatozoa ; ejaculated ; TESE ; MESA ; follow-up of children ; ART
• 刊名：Fertility and Sterility
• 出版年：2012
• 出版时间：February, 2012
• 年：2012
• 卷：97
• 期：2
• 页码：248-259
• 全文大小：455 K

文摘

Since the very early establishment of in?vitro insemination, it became clear that one of the limiting steps is the achievement of fertilization. Among the different assisted fertilization methods, intracytoplasmic sperm injection emerged as the ultimate technique to allow fertilization with ejaculated, epididymal, and testicular spermatozoa. This work describes the early steps that brought forth the development of intracytoplasmic sperm injection and its role in assisted reproductive techniques. The current methods to select the preferential male gamete will be elucidated and the concerns related to the offspring of severe male factor couples will be discussed.