Cell-mediated immune responses in the head-associated lymphoid tissues induced to a live attenuated avian coronavirus vaccine
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- 作者：Rucha S. Gurjar ; Stephen L. Gulley ; Frederik W. van Ginkel
- 关键词：IBV ; infectious bronchitis virus ; CALT ; conjunctiva-associated lymphoid tissue ; HG ; harderian gland ; IFN-¦Ã ; interferon gamma ; GZMA ; granzyme A ; CTL ; cytotoxic T lymphocyte ; EID50 ; median embryo infectious dose ; DPV ; days post vaccination ; TCM ; central me
- 刊名：Developmental and Comparative Immunology
- 出版年：2013
- 出版时间：December, 2013
- 年：2013
- 卷：41
- 期：4
- 页码：715-722
- 全文大小：488 K
文摘
Humoral immunity is important for controlling viral diseases of poultry, but recent studies have indicated that cytotoxic T cells also play an important role in the immune response to infectious bronchitis virus (IBV). To better understand the cell mediated immune responses to IBV in the mucosal and systemic immune compartments chickens were ocularly vaccinated with IBV. This induced a lymphocyte expansion in head-associated lymphoid tissues (HALT) and to a lesser extent in the spleen, followed by a rapid decline, probably due to homing of lymphocytes out of these organs and contraction of the lymphocyte population. This interpretation was supported by observations that changes in mononuclear cells were mirrored by that in CD3+CD44+ T cell abundance, which presumably represent T effector cells. Increased interferon gamma (IFN-¦Ã) expression was observed in the mucosal immune compartment, i.e., HALT, after primary vaccination, but shifted to the systemic immune compartment after boosting. In contrast, the expression of cytotoxicity-associated genes, i.e., granzyme A (GZMA) and perforin mRNA, remained associated with the HALT after boosting. Thus, an Ark-type IBV ocular vaccine induces a central memory IFN-¦Ã response in the spleen while the cytotoxic effector memory response, as measured by GZMA and perforin mRNA expression, remains associated with CALT after boosting.