Improvement of the liver pathology by the aqueous extract and the n-butanol fraction of Sida pilosa Retz in Schistosoma mansoni-infected mice

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• 作者：Hermine Boukeng Jatsa^a ; ^b ; ^c ; ^h ; ^{mjatsa@yahoo.fr" class="auth_mail" title="E-mail the corresponding author} ; Remo Castro Russo^b ; ^f ; ^{russorc@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Cintia Aparecida de Jesus Pereira^c ; ^{monjoloci@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Edenil Costa Aguilar^d ; ^{edenilcostaaguilar@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Cristiana Couto Garcia^b ; ^{criscoutog@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Emí ; lia Souza Araú ; jo^c ; ^{emilhotas@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Jailza Lima Rodrigues Oliveira^c ; ^{jailzalima@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Vanessa Fernandes Rodrigues^c ; ^{rfvannessa@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Viní ; cius Gustavo de Oliveira^c ; ^{vgo1990@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jacqueline Isaura Alvarez-Leite^d ; ^{jalvarezleite@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Fernã ; o Castro Braga^e ; ^{fernao@netuno.lcc.ufmg.br" class="auth_mail" title="E-mail the corresponding author} ; Louis-Albert Tchuem Tchuente^g ; ^h ; ^{tchuemtchuente@schisto.com" class="auth_mail" title="E-mail the corresponding author} ; Pierre Kamtchouing^a ; ^{pikam55@yahoo.fr" class="auth_mail" title="E-mail the corresponding author} ; Deborah Aparecida Negrã ; o-Corrê ; a^c ; ^{denegrao@icb.ufmg.br" class="auth_mail" title="E-mail the corresponding author} ; Mauro Martins Teixeira^b ; ^{mmtex@icb.ufmg.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sida pilosa ; Liver ; Oxidative stress ; Antioxidants ; Anti-fibrotic ; Schistosoma mansoni
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：2 March 2016
• 年：2016
• 卷：180
• 期：Complete
• 页码：114-123
• 全文大小：825 K

文摘

Sida pilosa Retz (Malvaceae) is a plant used in Africa for the treatment of intestinal helminthiasis, lower abdominal pains and dysmenorrhea.

Aim of the study

In order to determine the potential use of S. pilosa in the treatment of schistosomiasis mansoni, we evaluated the schistosomicidal, antioxidant and anti-fibrotic properties of the aqueous extract and the n-butanol fraction of its aerial parts.

Material and methods

S. pilosa aqueous extract (SpAE) at 100, 200 and 400 mg/kg and n-butanol fraction (SpBF) at 50, 100 and 200 mg/kg were administered per os to Schistosoma mansoni-infected mice for 4 weeks. Praziquantel (100 mg/kg×5 days) was used as reference drug. After sacrifice, worm burden and egg count, transaminases and proteins levels were evaluated. Malondialdehyde (MDA), lipid hydroperoxydes (LOOH), catalase (CAT), superoxide dismutase (SOD), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) were also measured. The anti-fibrotic effect of the plant was evaluated by the determination of hydroxyproline and γ-interferon (IFN-γ).

Results

The treatment of S. mansoni-infected mice by SpAE or SpBF resulted in a moderate reduction of worm burden and egg load in the liver and intestine. Both SpAE and SpBF significantly reversed the increasing liver proteins, MDA, LOOH and CAT levels induced by the infection. Moreover, SOD activity was improved by SpAE and SpBF. Schistosomiasis mansoni considerably increased the EPO (p<0.001) and MPO activities (p<0.001). SpAE treatment significantly reduced EPO and MPO activities at all doses. SpBF failed to reduce the increasing MPO and decreased EPO only at the highest dose. S. mansoni-infection induced an increase in hydroxyproline content (p<0.001) and a decrease in IFN-γ level (p<0.001). Both SpAE and SpBF significantly reduced hepatic hydroxyproline content, while only SpAE (p<0.05) improved IFN-γ level.

Conclusion

These results suggest that the liver pathology in schistosomiasis mansoni is improved by S. pilosa aqueous extract, which disclosed a moderate schistosomicidal, but strong antioxidant and anti-fibrotic activities. The n-butanol fraction was however less active than the aqueous extract.