Simultaneous determination of fraxin and its metabolite, fraxetin, in rat plasma by liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study
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- 作者：Haidong Wang ; Bingxin Xiao ; Zhiqiang Hao ; Zengxian Sun ; sunzx_715@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Fraxin ; Fraxetin ; Metabolite ; LC&ndash ; MS/MS ; Plasma
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：1017-1018
- 期：Complete
- 页码：70-74
- 全文大小：898 K
文摘
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An LC–MS/MS method was validated to determine fraxin and its metabolite fraxetin in rat plasma.
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The method has an LLOQ of 5.00 ng/mL for both analytes. Sample preparation was simple and quick with methanol protein precipitation.
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The method was applied to a pharmacokinetic study of fraxin in rats.