α-Galactosylceramide Induces Early B-Cell Activation through IL-4 Production by NKT Cells

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• 作者：Kitamura ; Hidemitsu ; Ohta ; Akio ; Sekimoto ; Masashi ; Sato ; Marimo ; Iwakabe ; Kenji ; Nakui ; Minoru ; et. al.
• 刊名：Cellular Immunology
• 出版年：2000
• 出版时间：January 10, 2000
• 年：2000
• 卷：199
• 期：1
• 页码：37-42
• 全文大小：116 K

文摘

α-Galactosylceramide (α-GalCer), a glycolipid antigen, specifically activates natural killer T (NKT) cells by a CD1d-restricted mechanism. In this work, we found that in vivo administration of α-GalCer resulted in the activation of B cells in addition to NKT cells, namely, α-GalCer administration caused upregulation of the early activation marker, CD69, on both NKT and B cells. In addition, expression of B7.2 and I-A^b on B cells was greatly upregulated by α-GalCer. However, serum levels of IgE, IgG1, and IgG2a were not significantly changed within 48 h. In the present experiments, it was also demonstrated that the upregulation of CD69 expression by α-GalCer was strongly blocked by anti-IL-4 monoclonal antibody. Moreover, B-cell activation by α-GalCer was not observed in NKT-deficient mice. These results suggested that antigen-stimulated NKT cells might play a critical role not only in early defense mechanisms but also in early B-cell activation through IL-4 production.