Preparation of 2,4-dichlorophenoxyacetic acid imprinted organic-inorganic hybrid monolithic column and application to selective solid-phase microextraction
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- 作者：Xiaofang Liu ; Quanfei Zhu ; Huaixia Chen ; hxch@hubu.edu.cn" class="auth_mail ; Liuzi Zhou ; Xueping Dang ; Jianlin Huang
- 关键词：2 ; 4-dichlorophenoxyacetic acid ; Organic-inorganic hybrid molecular imprinting monolith ; Solid-phase microextraction ; High-performance liquid chromatography
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：1 March, 2014
- 年：2014
- 卷：951-952
- 期：Complete
- 页码：32-37
- 全文大小：1020 K
文摘
An organic-inorganic hybrid molecular imprinting monolith (HMIM) has been prepared, characterized and applied for the determination of 2,4-dichlorophenoxyacetic acid (2,4-D)聽in rice with high-performance liquid chromatography-photodiodes array detector (HPLC-PAD). By optimizing the polymerization conditions, such as the volume ratio of the inorganic alcoholysate and organic part, the 2,4-D-HMIM was synthesized in a micro pipette tip using acrylamide as the functional monomer, ethylene dimethacrylate as the cross-linker and methanol as the porogenic solvent. The morphology of the monolith was studied by scanning electronmicroscopy and Fourier transform infrared spectra. The imprinted factor of the monolith for 2,4-D reached 3.29. A simple, rapid and sensitive method for the determination of 2,4-D in rice using the HMIM microextraction combined with high-performance liquid chromatography-photodiodes array detector was developed. Some parameters affecting the sample pretreatment were investigated, including the type and volume of eluent, the 铿俹w rate and volume of sample solution. The assay exhibited a linear dynamic range of 167-4167 渭g/kg with the correlation coefficient above 0.9972. The detection limit (at S/N = 3) was 50 渭g/kg. The proposed method was successfully applied for the selective determination of 2,4-D in rice.