C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice

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• 作者：Fang Fu^a ; Huabin Tian^a ; Xuesong Li^a ; ^b ; Yuekun Lang^a ; Guangzhi Tong^b ; Siguo Liu^a ; Haizhong Li^a ; Wei Wang^a ; Xi Li^a ; ^{lx2005@126.com} ; Xin Chen^a
• 关键词：Porcine circovirus type 2 ; DNA immunisation ; Capsid ; Mycobacterium tuberculosis ; C-terminal heat shock protein 70
• 刊名：The Veterinary Journal
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：195
• 期：2
• 页码：244-247
• 全文大小：524 K

文摘

Mycobacterium tuberculosis heat shock protein 70 (HSP70) and the peptide binding C-terminal portion of HSP70 (amino acids 359-610; HSP70c) exert an adjuvant effect when used in vaccines. To enhance the immunogenicity of a DNA vaccine against porcine circovirus type 2 (PCV2), recombinant plasmids encoding the PCV2 ORF2 (capsid) gene fused to full length hsp70 (pCA-TCH) or truncated C-terminal hsp70c (pCA-TCHc) were constructed. Immunisation of mice with pCA-TCHc induced higher serum immunoglobulin G antibody levels, stronger T helper 1 immune responses and lower PCV2 viral titres following challenge than immunisation with pCA-TCH or Cap plasmids only.