Identification and quantification of 3-iodothyronamine metabolites in mouse serum using liquid chromatography-tandem mass spectrometry

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• 作者：Sarah A. Hackenmueller ; Thomas S. Scanlan
• 关键词：3-Iodothyronamine ; Information dependent acquisition ; LC&ndash ; MS/MS
• 刊名：Journal of Chromatography A
• 出版年：2012
• 出版时间：21 September, 2012
• 年：2012
• 卷：1256
• 期：Complete
• 页码：89-97
• 全文大小：1068 K

文摘

3-Iodothyronamine (T₁AM) is an endogenous derivative of thyroxine. Recently there have been numerous reports of analytical methods to quantify endogenous T₁AM levels, but substantial discrepancies in concentration depending on the method of analysis (LC-MS/MS or immunoassay) suggest endogenous T₁AM may be covalently modified in vivo. Using information dependent acquisition methods to perform unbiased scans for T₁AM metabolites following a single IP injection in mice, we have identified O-sulfonate-T₁AM, N-acetyl-T₁AM and T₁AM-glucuronide as conjugates occurring in vivo, as well as the oxidatively deaminated 3-iodothyroacetic acid and non-iodinated thyroacetic acid. 3-iodothyroacetic acid, O-sulfonate-T₁AM and T₁AM-glucuronide are present in serum at greater concentrations that unmodified T₁AM and all metabolites are extensively distributed to tissues. These results suggest covalent modifications of T₁AM may play a critical role in regulating distribution and biological activity of T₁AM, and analytical methods to quantify endogenous T₁AM should be able to account for these metabolites as well.