- 作者：Eun-Jung Bak ; Jinmoon Kim ; Yun Hui Choi ; Ji-Hye Kim ; Dong-Eun Lee ; Gye-Hyeong Woo ; Jeong-Heon Cha ; Yun-Jung Yoo
- 关键词：Wogonin ; Hyperglycemia ; Hyperlipidemia ; Insulin ; PPAR ; Type 2 diabetes
- 刊名：Clinical Nutrition
- 出版年：February, 2014
- 年：2014
- 卷：33
- 期：1
- 页码：156-163
- 全文大小：2378 K
Summary
Background & aims
Wogonin is a flavonoid extracted from the root of Scutellaria baicalensis Gerogi. We evaluated the therapeutic effects of wogonin using db/db mice.Methods
Mice received wogonin or vehicle by oral gavage for 2 weeks. Blood glucose, insulin, and cholesterol levels were measured, and liver morphology was observed with histopathological analysis. The mRNA expression levels of PPAR伪, PPAR纬, and adiponectin in the liver and white adipose tissue (WAT) were determined by real-time PCR. Immunoblotting for AMPK and PPAR纬, and adipocyte differentiation were investigated in聽vitro using 3T3-L1 cells. A luciferase assay was used to measure PPAR伪 and PPAR纬 binding activity.
Results
The wogonin group showed decreased weight gain without a change in food intake and improved glucose tolerance. Serum insulin and cholesterol levels in the wogonin group were significantly decreased compared to those in the control group. The wogonin group also showed less accumulation of lipid droplets and glycogen in the liver. PPAR伪 and PPAR纬 expression levels in the liver and WAT and adiponectin expression level in WAT in the wogonin group were higher than those in the control group. In 3T3-L1 cells, wogonin was shown to stimulate AMPK activation in a dose-dependent manner. The presence of wogonin did not affect adipocyte differentiation or PPAR纬 protein level during adipogenesis. Notably, wogonin enhanced PPAR伪 but not PPAR纬 transactivation.
Conclusions
These indicate that wogonin may have beneficial effects on glucose and lipid metabolism related to enhanced PPAR伪 and adiponectin expression via AMPK activation. Importantly, wogonin did not cause deleterious effects, such as weight gain and fatty liver. Wogonin might be a useful therapeutic agent to treat type 2 diabetes.