Increased adenosine triphosphate production by peripheral blood CD4⁺ cells in patients with hematologic malignancies treated with stem cell mobilization agents

详细信息    查看全文

• 作者：Kiran Manga ; Geo Serban ; Joseph Schwartz ; Ronit Slotky ; Nita Patel ; Jianshe Fan ; Xiaolin Bai ; Ajai Chari ; David Savage ; Nicole Suciu-Foca ; Adriana I. Colovai
• 关键词：Stem cell transplantation ; Stem cell mobilization ; CD4 ⁺ T-cell activation ; Adenosine triphosphate production
• 刊名：Human Immunology
• 出版年：2010
• 出版时间：July 2010
• 年：2010
• 卷：71
• 期：7
• 页码：652-658
• 全文大小：1228 K

文摘

Hematopoietic stem cell (HSC) transplantation is an important therapeutic option for patients with hematologic malignancies. To explore the immunomodulatory effects of HSC mobilization agents, we studied the function and phenotype of CD4⁺ T cells from 16 adult patients with hematologic malignancies undergoing HSC mobilization treatment for autologous transplantation. Immune cell function was determined using the Immuknow (Cylex) assay by measuring the amount of adenosine triphosphate (ATP) produced by CD4⁺ cells from whole blood. ATP activity measured in G-CSF–treated patients was significantly higher than that measured in healthy individuals or “nonmobilized” patients. In patients treated with G-CSF, CD4⁺ T cells were predominantly CD25^lowFOXP3^low, consistent with an activated phenotype. However, T-cell depletion did not abrogate ATP production in blood samples from G-CSF–treated patients, indicating that CD4⁺ myeloid cells contributed to the increased ATP levels observed in these patients. There was a significant correlation between ATP activity and patient survival, suggesting that efficient activation of CD4⁺ cells during mobilization treatment predicts a low risk of disease relapse. Monitoring immune cell reactivity using the Immuknow assay may assist in the clinical management of patients with hematologic malignancies and optimization of HSC mobilization protocols.