MTERF1 Binds mtDNA to Prevent Transcriptional Interference at the Light-Strand Promoter but Is Dispensable for rRNA Gene Transcription Regulation

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• 作者：Mü ; gen Terzioglu¹ ; Benedetta Ruzzenente¹ ; ² ; Julia Harmel¹ ; Arnaud Mourier¹ ; Elisabeth Jemt³ ; Marcela Dá ; vila Ló ; pez³ ; Christian Kukat¹ ; James B. Stewart¹ ; Rolf Wibom⁴ ; Caroline Meharg⁵ ; Bianca Habermann¹ ; Maria Falkenberg³ ; Claes M. Gustafsson³ ; Chan Bae Park¹ ; ⁶ ; ^{chanbaepark@ajou.ac.kr} ; Nils-Gö ; ran Larsson¹ ; ⁴ ; ^{larsson@age.mpg.de}
• 刊名：Cell Metabolism
• 出版年：2013
• 出版时间：2 April, 2013
• 年：2013
• 卷：17
• 期：4
• 页码：618-626
• 全文大小：981 K

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Summary

Mitochondrial transcription termination factor 1, MTERF1, has been reported to couple rRNA gene transcription initiation with termination and is therefore thought to be a key regulator of mammalian mitochondrial ribosome biogenesis. The prevailing model is based on a series of observations published over the last two decades, but no in?vivo evidence exists to show that MTERF1 regulates transcription of the heavy-strand region of mtDNA containing the rRNA genes. Here, we demonstrate that knockout of Mterf1 in mice has no effect on mitochondrial rRNA levels or mitochondrial translation. Instead, loss of Mterf1 influences transcription initiation at the light-strand promoter, resulting in a decrease of?de novo transcription manifested as reduced 7S?RNA levels. Based on these observations, we suggest that MTERF1 does not regulate heavy-strand transcription, but rather acts to block transcription on the opposite strand of mtDNA to prevent transcription interference at the light-strand promoter.