UHRF1 recruits the histone acetyltransferase Tip60 and controls its expression and activity
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- 作者：Mayada Achour ; Guy Fuhrmann ; Mahmoud Alhosin ; Philippe Rondé ; Thierry Chataigneau ; Marc Mousli ; Valé ; rie B. Schini-Kerth ; Christian Bronner
- 关键词：DNMT1 ; Epigenetic ; Histone H2A ; Tip60 ; UHRF1
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2009
- 出版时间：18 December 2009
- 年：2009
- 卷：390
- 期：3
- 页码：523-528
- 全文大小：513 K
文摘
Tat-interactive protein, 60 kDa (Tip60) is a histone acetyltransferase with specificity toward lysine 5 of histone H2A (H2AK5) and plays multiple roles in chromatin remodeling processes. Co-immunoprecipitation experiments performed on Jurkat cells, showed that Tip60 is present in the same macro-molecular complex as UHRF1 (Ubiquitin-like containing PHD and RING domain 1), DNMT1 (DNA methyltransferase 1), and HDAC1 (histone deacetylase 1). Furthermore, immunocytochemistry experiments confirmed that Tip60 co-localizes with the UHRF1/DNMT1 complex. Although down-regulation of UHRF1 by RNA interference enhanced Tip60 expression, a significant decrease of the level of acetylated H2AK5 was observed. Consistently, we have observed that down-regulation of Tip60 and DNMT1 by RNA interference, dramatically reduced the levels of acetylated H2AK5. Altogether, these results suggest that Tip60 is a novel partner of the epigenetic integration platform interplayed by UHRF1, DNMT1 and HDAC1 involved in the epigenetic code replication.