Electrochemical uranyl cation biosensor with DNA oligonucleotides as receptor layer

详细信息    查看全文

• 作者：Marta Jarczewska ; Robert Zi贸艂kowski ; 艁ukasz G贸rski ; El偶bieta Malinowska
• 关键词：DNA biosensor ; Electrochemical biosensor ; Uranyl cation ; Metals determination ; Mixed monolayer
• 刊名：Bioelectrochemistry
• 出版年：April, 2014
• 年：2014
• 卷：96
• 期：Complete
• 页码：1-6
• 全文大小：420 K

文摘

The present study aims at the further development of the uranyl oligonucleotide-based voltammetric biosensor, which takes advantage of strong interaction between UO₂^2 + and phosphate DNA backbone. Herein we report the optimization of working parameters of previously elaborated electrochemical DNA biosensor. It is shown that the sensor sensitivity is highly dependent on the oligonucleotide probe length and the incubation time of sensor in a sample solution. Consequently, the highest sensitivity was obtained for 10-nucleotide sequence and 60 min incubation time. The lower detection limit towards uranyl cation for developed biosensor was 30 nM. The influence of mixed monolayers and the possibility of developing a non-calibration device were also investigated. The selectivity of the proposed biosensor was significantly improved via elimination of adenine nucleobases from the DNA probe. Moreover, the regeneration procedure was elaborated and tested to prolong the use of the same biosensor for 4 subsequent determinations of UO₂^2 +.