Manganese porphyrins 鈥?Studies on their potential use for protein labeling

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• 作者：Kamila Konopi艅ska ; Mariusz Pietrzak ; ^{mariusz@ch.pw.edu.pl" class="auth_mail} ; El偶bieta Malinowska
• 关键词：Labeling ; Metalloporphyrin ; Affinity biosensor ; Conjugate
• 刊名：Microchemical Journal
• 出版年：July, 2014
• 年：2014
• 卷：115
• 期：Complete
• 页码：1-5
• 全文大小：753 K

文摘

The presented work aims at characterization of electrochemical and optical behavior of 5,10,15,20-Tetraphenyl-21H, 23H-porphyrin manganese(III) chloride (Mn-tpp) in terms of its potential usage as a hybrid label for particles of biological origin. The results demonstrate the possibility of Mn-tpp application in triple detection system, as it was proved to be successfully determined in presence of proteins by means of differential pulse voltammetry, UV-Vis spectrophotometry and spectrofluorimetry.

In case of electrochemical detection, a variety of parameters that may affect the nature of the generated signal was optimized. The selection of supporting electrolyte was also taken into consideration with particular attention, employing tetrabutylammonium salts with different counterions (borate, bromide, iodide and perchlorate).

Subsequently, the efforts were undertaken to examine the protein's (mimicking the receptor or surface blocking agent) impact on the derived signals. In the role of model bioparticles bovine serum albumin (BSA), chicken egg albumin (CEA) and immunoglobulin G (IgG) were employed. After addition of protein, displacement of received signals along the potential axis (a shift of the potential) and changes in signals intensities were observed.

Simultaneously, research on Mn-tpp was carried out using spectroscopic techniques in order to assess its suitability for use as a label also in optical detection mode.