Designing functionalized gold surfaces and nanostructures for Laser Desorption Ionisation Mass Spectrometry

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• 作者：Rosa Pilolli ; Nicoletta Ditaranto ; Antonio Monopoli ; Angelo Nacci ; Francesco Palmisano ; Luigia Sabbatini ; Nicola Cioffi
• 关键词：Gold nanoparticles ; Surface functionalization ; XPS ; MALDI-MS
• 刊名：Vacuum
• 出版年：2014
• 出版时间：February, 2014
• 年：2014
• 卷：100
• 期：Complete
• 页码：78-83
• 全文大小：752 K

文摘

The present work is aimed at developing gold nanostructures functionalized with antenna systems to exploit the synergistic nanostructure/antenna desorption-ionization efficiency. A potential Matrix-Assisted Laser Desorption Ionisation (MALDI) organic matrix has been modified introducing specific functional groups or molecular linker and used as a capping agent for gold nanostructures.

In particular, conjugated naphthyl-thio-derivative, i.e. 4-mercaptonaphthalene-1,8-dicarboxylic acid, was synthesized and characterized by means of nuclear magnetic resonance, UV-visible and X-ray photoelectron spectroscopies. Afterwards, the thio-derivative was used as covalent surface modifier for flat gold surfaces and nanostructured gold films. These surfaces were thoroughly characterized by means of parallel angle-resolved X-ray photoelectron spectroscopy to obtain quantitative information about elemental composition, chemical speciation, and in-depth distribution of the target chemical functional groups. Finally the compound was preliminarily tested as a non-conventional matrix in Laser Desorption Ionisation Mass Spectrometry (LDI-MS) analysis of low molecular weight biomolecules in order to assess its capability of acting as the antenna system and proton donor after covalent bonding to gold nanomaterials.