Characterization of pikeperch (Sander lucioperca) milt collected with a syringe and a catheter

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• 作者：Beata Sarosiek^a ; ^{b.sarosiek@pan.olsztyn.pl" class="auth_mail" title="E-mail the corresponding author} ; Katarzyna Dryl^a ; S艂awomir Krejszeff^b ; Daniel 呕arski^b ; ^c
• 关键词：Sander ; Milt ; Catheter ; Urine contamination ; Motility
• 刊名：Aquaculture
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：450
• 期：Complete
• 页码：14-16
• 全文大小：255 K

文摘

Collection of fish milt by stripping risks contamination with urine, which may negatively influence milt characteristics and quality. Urine contamination of milt may be avoided by using a catheter for sperm collection. The objective of this study was to compare basic pikeperch milt parameters, i.e., sperm concentration (SC), pH, osmolality of seminal plasma (OSM), and motility parameters of spermatozoa analyzed with a CASA system (Computer Assisted Sperm Analysis), collected either with a syringe (as the standard method of sperm collection) or with a catheter. Every parameter of pikeperch milt collected with a catheter was significantly higher (P < 0.05; SC = 12.31 &plusmn; 3.81 × 10⁹ ml^− 1, pH = 9.04 &plusmn; 0.07, OSM = 311 &plusmn; 16 mOsm kg^− 1) than that collected with a syringe (SC = 8.05 &plusmn; 3.04 × 10⁹ ml^− 1, pH = 8.93 &plusmn; 0.09, OSM = 228 &plusmn; 51 mOsm kg^− 1), which clearly suggests urine contamination of milt collected with the standard procedure. Moreover, milt collected with catheter was characterized by higher spermatozoa motility parameters, e.g., motility rate of sperm collected with a catheter was 73% whereas the motility rate of sperm collected with a syringe did not exceed 35%. The results obtained in this work indicate that the milt collected with the standard procedure is likely to be exposed to contamination with urine, which may cause a significant reduction of quality parameters. Collection of sperm with a catheter was proven to effectively reduce the contamination of sperm with urine. Therefore, it is recommended to collect pikeperch sperm using a catheter.